Laboratory of Analytical Chemistry and Science of Separations


The laboratory deals with fundamental and applicative research topics in the field of Analytical Chemistry, with particular reference to Bioanalytical Chemistry and Separation Science. Prototype level instrumentation and original analytical methods are developed in the clinical, pharmaceutical, industrial and materials sectors.



FFF system with LS Wyatt Technologies Eclipse probe

Flow-field fractionation (FlFFF) is a class of techniques capable of separating analytes based on the hydrodynamic dimension. The technique allows the separation of complex colloidal mixtures under native conditions.  Downstream of the FlFFF device, a series of online detectors (refraction index, UV-Vis spectrophotometer, laser scattering) allow the simultaneous characterization of the analytes during the analysis.

HPLC Agilent Technologies 1200 series UV-Vis DAD e FLD e 1260Infinity series UV-Vis DAD

The instruments allow the separation and quantitative analysis of organic components in a mixture.

UV-Vis Spectrophotometer Agilent Technologies Cary 50

The instrument allows the registration of absorption spectra of substances in the spectral range between 200 and 800 nm. It also allows quantitative analyzes to be performed by measuring the single wavelength absorbance and data interpolation on the appropriate calibration curve. The instrument can be used to perform kinetic measurements.

Multiskan EX Thermo Electron Corporation

Plate reader for microtiter plates. Filters 405nm, 450nm, 620nm.
The instrument allows absorbance measurements at wavelengths indicated both as a single reading and in kinetic mode

Lyophilizer Alpha 1-2 LDplus CHRIST

The instrument allows lyophilizing samples in aqueous solution contained in Eppendorf, Falcon (15ml, 50 ml) and 100ml glass flasks.

Atomic absorption Analyst 400 Perkin Elmer

The instrument allows to perform the quantitative analysis of metals in aqueous solutions. Analyzes can be performed using both the FAAS and ETAAS techniques applicable respectively to trace (ppm) and ultratrace (ppb) quantification of the analytes of interest.


The laboratory carries out commissioned analysis services and industrial research in the field of analytical chemistry. 


Prof. Barbara Roda
Dr. Sonia Casolari

Teaching and training activity

The instrumentation supplied with the laboratory is used for exercises in Analytical Chemistry for the study courses in Chemistry and Chemistry of Materials (degree), Chemistry (Master's Degree), Biology of Health (LM)
The instrumentation is also used for the development of degree theses in the same degree programs and for research projects of doctoral students in Chemical Sciences and post-doc.